Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/9493
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dc.contributor.authorBalachandran, W-
dc.contributor.authorMcKay, R-
dc.contributor.authorCrow, P-
dc.contributor.authorManoharanehru, B-
dc.contributor.author4th Micro and Nano Flows Conference (MNF2014)-
dc.coverage.spatialUCL, London, UK-
dc.date.accessioned2014-12-11T15:39:16Z-
dc.date.available2014-09-07-
dc.date.available2014-12-11T15:39:16Z-
dc.date.issued2014-
dc.identifier.citation4th Micro and Nano Flows Conference, University College London, UK, 7-10 September 2014, Editors CS König, TG Karayiannis and S. Balabanien_US
dc.identifier.isbn978-1-908549-16-7-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/9493-
dc.descriptionThis paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute, ASME Press, LCN London Centre for Nanotechnology, UCL University College London, UCL Engineering, the International NanoScience Community, www.nanopaprika.eu.en_US
dc.description.abstractBrunel DoCLab is part of the esti2 consortium developing electronic self-testing instruments for sexually transmitted infections using nucleic acid amplification testing (NAAT). A proprietary sample collection device has been designed to integrate directly with a microfluidic cartridge. Cell lysis was conducted using a chemical method and nucleic acid purification was done on an activated cellulose membrane. The microfluidic device incorporates passive mixing of the lysis-binding buffers and sample. Preliminary results have shown extraction efficiencies for this new membrane of 69% and 57% compared to the commercial Qiagen extraction method of 85% and 59.4% for 0.1ng/μL and 100ng/μL salmon sperm DNA spiked in phosphate buffered solution. Preliminary extraction experiments in the passive mixer cartridges with lysis and nucleic acid purification showed extraction efficiency around 80% of the commercial Qiagen kit. Isothermal amplification was conducted using thermophillic helicase dependant amplification. A low cost benchtop real-time isothermal amplification platform has been developed capable of running six amplifications simultaneously. Work to integrate sample collection, nucleic acid extraction and isothermal amplification is currently underway.en_US
dc.language.isoenen_US
dc.publisherBrunel University Londonen_US
dc.relation.ispartofseriesID 211-
dc.source4th Micro and Nano Flows Conference-
dc.source4th Micro and Nano Flows Conference-
dc.subjectMicrofluidicsen_US
dc.subjectMicroengineeringen_US
dc.subjectPoint-of-careen_US
dc.subjectMolecular diagnosticsen_US
dc.titleSample preparation for point of care molecular diagnostics of STIsen_US
dc.typeConference Paperen_US
pubs.finish-date2014-09-10-
pubs.finish-date2014-09-10-
pubs.start-date2014-09-07-
pubs.start-date2014-09-07-
pubs.organisational-data/Brunel-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences/Dept of Mechanical, Aerospace and Civil Engineering-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences/Dept of Mechanical, Aerospace and Civil Engineering/Mechanical and Aerospace Engineering-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme/Institute of Energy Futures-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme/Institute of Energy Futures/Energy Efficient and Sustainable Technologies-
pubs.organisational-data/Brunel/University Research Centres and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/Brunel Business School - URCs and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/Brunel Business School - URCs and Groups/Centre for Research into Entrepreneurship, International Business and Innovation in Emerging Markets-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute for Ageing Studies-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute of Cancer Genetics and Pharmacogenomics-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Systems and Synthetic Biology-
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