Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/9232
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dc.contributor.authorShtam, TA-
dc.contributor.authorKovalev, RA-
dc.contributor.authorVarfolomeeva, EY-
dc.contributor.authorMakarov, EM-
dc.contributor.authorKil, YV-
dc.contributor.authorFilatov, MV-
dc.date.accessioned2014-11-25T11:42:15Z-
dc.date.available2013-11-18-
dc.date.available2014-11-25T11:42:15Z-
dc.date.issued2013-
dc.identifier.citationCell Communication and Signaling, 11:88 (18 November 2013)en_US
dc.identifier.issn1478-811X-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/9232-
dc.descriptionThis is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.description.abstractBackground: Exosomes are nano-sized vesicles of endocytic origin that are involved in cell-to-cell communication including shuttle RNA, mainly mRNA and microRNA. As exosomes naturally carry RNA between cells, these particles might be useful in gene cancer therapy to deliver therapeutic short interfering RNA (siRNA) to the target cells. Despite the promise of RNA interference (RNAi) for use in therapy, several technical obstacles must be overcome. Exogenous siRNA is prone to degradation, has a limited ability to cross cell membranes and may induce an immune response. Naturally occurring RNA carriers, such as exosomes, might provide an untapped source of effective delivery strategies. Results: This study demonstrates that exosomes can deliver siRNA to recipient cells in vitro. The different strategies were used to introduce siRNAs into human exosomes of various origins. The delivery of fluorescently labeled siRNA via exosomes to cells was confirmed using confocal microscopy and flow cytometry. Two different siRNAs against RAD51 and RAD52 were used to transfect into the exosomes for therapeutic delivery into target cells. The exosome-delivered siRNAs were effective at causing post-transcriptional gene silencing in recipient cells. Moreover, the exosome-delivered siRNA against RAD51 was functional and caused the massive reproductive cell death of recipient cancer cells. Conclusions: The results strongly suggest that exosomes effectively delivered the siRNA into the target cells. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated in vitro by the strong knockdown of RAD51, a prospective therapeutic target for cancer cells. The results give an additional evidence of the ability to use human exosomes as vectors in cancer therapy, including RNAi-based gene therapy. © 2013 Shtam et al.; licensee BioMed Central Ltd.en_US
dc.description.sponsorshipRussian Federal Program “Scientific and Scientific-Pedagogical Personnel of Innovative Russia”, contract 14.740.11.0754 and Fellowship from the Administration of Leningrad region to Shtam T.en_US
dc.languageeng-
dc.language.isoenen_US
dc.subjectCancer therapyen_US
dc.subjectDrug delivery systemen_US
dc.subjectExosomesen_US
dc.subjectRAD51en_US
dc.subjectRNA interference (RNAi)en_US
dc.titleExosomes are natural carriers of exogenous siRNA to human cells in vitroen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1186/1478-811X-11-88-
dc.relation.isPartOfCell Communication and Signaling-
dc.relation.isPartOfCell Communication and Signaling-
pubs.issue1-
pubs.issue1-
pubs.volume11-
pubs.volume11-
pubs.organisational-data/Brunel-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences/Biological Sciences-
pubs.organisational-data/Brunel/University Research Centres and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute for Ageing Studies-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute of Cancer Genetics and Pharmacogenomics-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Systems and Synthetic Biology-
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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