Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/8899
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dc.contributor.authorSiu, YL-
dc.contributor.authorTeoh, KT-
dc.contributor.authorLo, J-
dc.contributor.authorChan, CM-
dc.contributor.authorKien, F-
dc.contributor.authorEscriou, N-
dc.contributor.authorTsao, SW-
dc.contributor.authorNicholls, JM-
dc.contributor.authorAltmeyer, R-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorBruzzone, R-
dc.contributor.authorNal, B-
dc.date.accessioned2014-08-18T13:58:04Z-
dc.date.available2014-08-18T13:58:04Z-
dc.date.issued2008-
dc.identifier.citationJournal of Virology, 82(22), 11318 - 11330, 2008en_US
dc.identifier.issn0022-538X-
dc.identifier.urihttp://jvi.asm.org/content/82/22/11318en
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/8899-
dc.descriptionCopyright @ 2008 American Society for Microbiology.en_US
dc.description.abstractThe production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.en_US
dc.description.sponsorshipThe University of Hong Kong and the French Ministry of Health.en_US
dc.languageEnglish-
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.subjectCoronavirusen_US
dc.subjectSARS-CoVen_US
dc.subjectM proteinsen_US
dc.subjectE proteinsen_US
dc.subjectN proteinsen_US
dc.titleThe M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particlesen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.01052-08-
pubs.organisational-data/Brunel-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences/Biological Sciences-
pubs.organisational-data/Brunel/University Research Centres and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute for Ageing Studies-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute of Cancer Genetics and Pharmacogenomics-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Systems and Synthetic Biology-
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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