Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/8736
Title: Pathological replication in cells lacking RecG DNA translocase
Authors: Rudolph, CJ
Upton, AL
Harris, L
Lloyd, RG
Keywords: DNA replication;Replication forks;RecG helicase;Escherichia coli
Issue Date: 2009
Publisher: Wiley-Blackwell
Citation: Molecular Microbiology, 73(3), 352 - 366, 2009
Abstract: Little is known about what happens when forks meet to complete DNA replication in any organism. In this study we present data suggesting that the collision of replication forks is a potential threat to genomic stability. We demonstrate that Escherichia coli cells lacking RecG helicase suffer major defects in chromosome replication following UV irradiation, and that this is associated with high levels of DNA synthesis initiated independently of the initiator protein DnaA. This UV-induced stable DNA replication is dependent on PriA helicase and continues long after UV-induced lesions have been excised. We suggest UV irradiation triggers the assembly of new replication forks, leading to multiple fork collisions outside the terminus area. Such collisions may generate branched DNAs that serve to establish further new forks, resulting in uncontrolled DNA amplification. We propose that RecG reduces the likelihood of this pathological cascade being set in motion by reducing initiation of replication at D- and R-loops, and other structures generated as a result of fork collisions. Our results shed light on why replication initiation in bacteria is limited to a single origin and why termination is carefully orchestrated to a single event within a restricted area each cell cycle.
Description: This is an open access article, shared under a Creative Commons licence. Copyright © 2009 The Authors.
URI: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2009.06773.x/abstract
http://bura.brunel.ac.uk/handle/2438/8736
DOI: http://dx.doi.org/10.1111/j.1365-2958.2009.06773.x
ISSN: 0950-382X
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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