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Title: In situ off-axis holography of marine plankton
Authors: Krantz, EP
Watson, J
Hobson, PR
Lampitt, RS
Rogerson, A
Keywords: Holography;Off-axis holography;Particle holography;Plankton
Issue Date: 1996
Publisher: SPIE
Citation: Proceedings of SPIE. 2652(1996) 236-243
Abstract: We present an off-axis transmission holographic technique for recording marine plankton in situ within a test tank of 36,000 ml with a pulsed laser in a 40 ns interval. The holographic plate is located in air and is therefore unaffected by aquatic conditions that may cause emulsion degradation (e.g. non-uniform swelling and surface contamination). The reference beam traverses a path in air only, and thus remains unaffected by dense concentrations of plankton. Third order aberrations, notably spherical aberration and astigmatism, are suppressed to yield an experimental resolution of 7 lp/mm (70 micrometres) with a USAF 1951 target located 600 mm in water from the observation window. Plankton particle counts examined by real image reconstruction show a strong correlation with duplicate samples examined under a microscope.
ISSN: 0-8194-2026-3
Appears in Collections:Electronic and Computer Engineering
Dept of Electronic and Computer Engineering Research Papers

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