Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/12656
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMillet, JK-
dc.contributor.authorNal, B-
dc.contributor.editorMaier, HJ-
dc.contributor.editorBickerton, E-
dc.contributor.editorBritton, P-
dc.date.accessioned2016-05-20T11:51:00Z-
dc.date.available2016-05-20T11:51:00Z-
dc.date.issued2015-
dc.identifier.citationCoronaviruses, Methods in Molecular Biology, vol. 1282, pp. 231 - 240, (2015)en_US
dc.identifier.isbn978-1-4939-2437-0-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://link.springer.com/protocol/10.1007%2F978-1-4939-2438-7_19-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/12656-
dc.description.abstractSince its identification in the 1990s, the RNA interference (RNAi) pathway has proven extremely useful in elucidating the function of proteins in the context of cells and even whole organisms. In particular, this sequence-specific and powerful loss-of-function approach has greatly simplified the study of the role of host cell factors implicated in the life cycle of viruses. Here, we detail the RNAi method we have developed and used to specifically knock down the expression of ezrin, an actin binding protein that was identified by yeast two-hybrid screening to interact with the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) spike (S) protein. This method was used to study the role of ezrin, specifically during the entry stage of SARS-CoV infection.en_US
dc.format.extent231 - 240-
dc.language.isoenen_US
dc.publisherSpringer New Yorken_US
dc.subjectRNA interference (RNAi)en_US
dc.subjectSmall interfering RNA (siRNA)en_US
dc.subjectEzrinen_US
dc.subjectSevere Acute Respiratory Syndrome Coronavirus (SARS-CoV)en_US
dc.subjectVirusen_US
dc.subjectHost interactionsen_US
dc.titleInvestigation of the functional roles of host cell proteins involved in Coronavirus infection using highly specific and scalable RNA interference (RNAi) approachen_US
dc.typeBook chapteren_US
dc.identifier.doihttp://dx.doi.org/10.1007/978-1-4939-2438-7_19-
dc.relation.isPartOfCoronaviruses-
dc.relation.isPartOfMethods in Molecular Biology-
pubs.edition2015-
pubs.publication-statusPublished-
pubs.volume1282-
Appears in Collections:Dept of Life Sciences Research Papers

Files in This Item:
File Description SizeFormat 
Fulltext.pdf344.3 kBAdobe PDFView/Open


Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.