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Title: 25 years on and no end in sight: a perspective on the role of RecG protein.
Authors: Lloyd, RG
Rudolph, CJ
Keywords: Stalled replication forks;Homologous recombination;Stable DNA replication;SDR;Replication termination;Replication fork collisions
Issue Date: 2016
Publisher: Springer Berlin Heidelberg
Citation: Current Genetics, 62(4): pp. 827 -840, (2016)
Abstract: The RecG protein of Escherichia coli is a double-stranded DNA translocase that unwinds a variety of branched substrates in vitro. Although initially associated with homologous recombination and DNA repair, studies of cells lacking RecG over the past 25 years have led to the suggestion that the protein might be multi-functional and associated with a number of additional cellular processes, including initiation of origin-independent DNA replication, the rescue of stalled or damaged replication forks, replication restart, stationary phase or stress-induced 'adaptive' mutations and most recently, naïve adaptation in CRISPR-Cas immunity. Here we discuss the possibility that many of the phenotypes of recG mutant cells that have led to this conclusion may stem from a single defect, namely the failure to prevent re-replication of the chromosome. We also present data indicating that this failure does indeed contribute substantially to the much-reduced recovery of recombinants in conjugational crosses with strains lacking both RecG and the RuvABC Holliday junction resolvase.
ISSN: 0172-8083
Appears in Collections:Dept of Life Sciences Research Papers

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