Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/11283
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dc.contributor.authorBai, B-
dc.contributor.authorCai, X-
dc.contributor.authorJiang, Y-
dc.contributor.authorKarteris, E-
dc.contributor.authorChen, J-
dc.contributor.authorChen, J-
dc.date.accessioned2015-08-25T14:19:37Z-
dc.date.available2014-10-
dc.date.available2015-08-25T14:19:37Z-
dc.date.issued2014-
dc.identifier.citationJournal of Cellular and Molecular Medicine, 2014, 18 (10), pp. 2071 - 2081en_US
dc.identifier.issn1582-1838-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/11283-
dc.description.abstractDimerization of G protein-coupled receptors (GPCRs) is crucial for receptor function including agonist affinity, efficacy, trafficking and specificity of signal transduction, including G protein coupling. Emerging data suggest that the cardiovascular system is the main target of apelin, which exerts an overall neuroprotective role, and is a positive regulator of angiotensin-converting enzyme 2 (ACE2) in heart failure. Moreover, ACE2 cleaves off C-terminal residues of vasoactive peptides including apelin-13, and neurotensin that activate the apelin receptor (APJ) and neurotensin receptor 1 (NTSR1) respectively, that belong to the A class of GPCRs. Therefore, based on the similar mode of modification by ACE2 at peptide level, the homology at amino acid level and the capability of forming dimers with other GPCRs, we have been suggested that APJ and NTSR1 can form a functional heterodimer. Using co-immunoprecipitation, BRET and FRET, we provided conclusive evidence of heterodimerization between APJ and NTSR1 in a constitutive and induced form. Upon agonist stimulation, hetrodimerization enhanced ERK1/2 activation and increased proliferation via activation of Gq α-subunits. These novel data provide evidence for a physiological role of APJ/NTSR1 heterodimers in terms of ERK1/2 activation and increased intracellular calcium and induced cell proliferation and provide potential new pharmaceutical targets for cardiovascular disease. © 2014 The Authors.en_US
dc.format.extent2071 - 2081-
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectG protein-coupled receptoren_US
dc.subjectNeurotensin receptor 1en_US
dc.subjectApelin receptoren_US
dc.subjectHeterodimerizationen_US
dc.subjectResonance energy transferen_US
dc.subjectERK 1/2en_US
dc.titleHeterodimerization of apelin receptor and neurotensin receptor 1 induces phosphorylation of ERK1/2 and cell proliferation via Gαq-mediated mechanismen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1111/jcmm.12404-
dc.relation.isPartOfJournal of Cellular and Molecular Medicine-
pubs.issue10-
pubs.publication-statusPublished-
pubs.publication-statusPublished-
pubs.volume18-
Appears in Collections:Dept of Life Sciences Research Papers

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