Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/11037
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dc.contributor.authorMouratidis, PX-
dc.contributor.authorGeorge, AJ-
dc.coverage.spatialNew Zealand-
dc.coverage.spatialNew Zealand-
dc.date.accessioned2015-06-22T12:20:23Z-
dc.date.available2015-
dc.date.available2015-06-22T12:20:23Z-
dc.date.issued2015-
dc.identifier.citationJournal of Inflammation Research, 2015: 8, 97–106, (21 May 2015)en_US
dc.identifier.issn1178-7031-
dc.identifier.urihttp://www.dovepress.com/regulation-of-indoleamine-23-dioxygenase-in-primary-human-saphenous-ve-peer-reviewed-article-JIR-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/11037-
dc.description.abstractBACKGROUND: Indoleamine 2,3-dioxygenase (IDO) is an enzyme associated with the regulation of immune responses. Cytokines such as IFNγ induce its expression in endothelial cells originating from immune-privileged sites. In this study, we investigate regulators of IDO in primary endothelial cells from a non-immune-privileged site and determine whether IDO expression affects immune cell behavior. METHODS: IDO expression was determined using real-time quantitative polymerase chain reaction and immunoblotting. IDO activity was estimated using an IDO enzyme assay. Primary cells were transfected using microporation, and T-cell migration was determined using a cell transmigration assay. RESULTS: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ but not after treatment with TNFα, IL-1β, IL-2, IL-4, IL-6, or IL-10. VEGFβ and heparin negatively regulate IFNγ-driven increases in IDO. Overexpression of IDO in endothelial cells does not affect transmigration of T-cells. CONCLUSION: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ. Heparin and angiogenesis stimulators such as VEGFβ negatively regulate its expression.en_US
dc.description.sponsorshipThe British Heart Foundation (BHF).en_US
dc.format.extent97 - 106-
dc.languageeng-
dc.language.isoenen_US
dc.publisherDove Press Ltden_US
dc.subjectHSVECen_US
dc.subjectIFNγen_US
dc.subjectVEGFβen_US
dc.subjectEndotheliumen_US
dc.subjectInflammationen_US
dc.titleRegulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells.en_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.2147/JIR.S82202-
dc.relation.isPartOfJ Inflamm Res-
pubs.volume8-
pubs.volume8-
Appears in Collections:Dept of Clinical Sciences Research Papers

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