Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/10835
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dc.contributor.authorLockyer, AE-
dc.contributor.authorNoble, LR-
dc.contributor.authorRollinson, D-
dc.contributor.authorJones, CS-
dc.date.accessioned2015-05-12T13:39:40Z-
dc.date.available2005-05-
dc.date.available2015-05-12T13:39:40Z-
dc.date.issued2005-
dc.identifier.citationMemorias do Instituto Oswaldo Cruz, 100 (3): 259 - 262, (2005)en_US
dc.identifier.issn0074-0276-
dc.identifier.urihttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300007-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/10835-
dc.description.abstractCytochrome p450s (cyp450s) are a family of structurally related proteins, with diverse functions, including steroid synthesis and breakdown of toxins. This paper reports the full-length sequence of a novel cyp450 gene, the first to be isolated from the tropical freshwater snail Biomphalaria glabrata, an important intermediate host of Schistosoma mansoni. The nucleotide sequence is 2291 bp with a predicted amino acid sequence of 584aa. The sequence demonstrates conserved cyp450 structural motifs, but is sufficiently different from previously reported cyp450 sequences to be given a new classification, CYP320A1. Initially identified as down-regulated in partially resistant snails in response to S. mansoni infection, amplification of this gene using RT-PCR in both totally resistant or susceptible snail lines when exposed to infection, and all tissues examined, suggests ubiquitous expression. Characterization of the first cyp450 from B. glabrata is significant in understanding the evolution of these metabolically important proteins.en_US
dc.format.extent259 - 262-
dc.languageeng-
dc.language.isoenen_US
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúdeen_US
dc.subjectBiomphalaria glabrataen_US
dc.subjectCYP320A1en_US
dc.subjectcyp450en_US
dc.subjectCytochrome p450en_US
dc.subjectRACEen_US
dc.subjectSchistosoma mansonien_US
dc.titleIsolation and characterization of the full-length cDNA encoding a member of a novel cytochrome p450 family (CYP320A1) from the tropical freshwater snail, Biomphalaria glabrata, intermediate host for Schistosoma mansonien_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1590/S0074-02762005000300007-
dc.relation.isPartOfMemorias do Instituto Oswaldo Cruz-
pubs.issue3-
pubs.volume100-
Appears in Collections:Dept of Life Sciences Research Papers

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