Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/10500
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dc.contributor.authorLynch, AE-
dc.contributor.authorTriajianto, J-
dc.contributor.authorRoutledge, E-
dc.date.accessioned2015-03-24T15:36:00Z-
dc.date.available2014-08-14-
dc.date.available2015-03-24T15:36:00Z-
dc.date.issued2014-
dc.identifier.citationPLoS ONE, 2014, 9 (8)en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/10500-
dc.descriptionThis article has been made available through the Brunel Open Access Publishing Fund.-
dc.description.abstractDirect visualisation of cells for the purpose of studying their motility has typically required expensive microscopy equipment. However, recent advances in digital sensors mean that it is now possible to image cells for a fraction of the price of a standard microscope. Along with low-cost imaging there has also been a large increase in the availability of high quality, open-source analysis programs. In this study we describe the development and performance of an expandable cell motility system employing inexpensive, commercially available digital USB microscopes to image various cell types using time-lapse and perform tracking assays in proof-of-concept experiments. With this system we were able to measure and record three separate assays simultaneously on one personal computer using identical microscopes, and obtained tracking results comparable in quality to those from other studies that used standard, more expensive, equipment. The microscopes used in our system were capable of a maximum magnification of 413.6x. Although resolution was lower than that of a standard inverted microscope we found this difference to be indistinguishable at the magnification chosen for cell tracking experiments (206.8x). In preliminary cell culture experiments using our system, velocities (mean mm/min ± SE) of 0.81±0.01 (Biomphalaria glabrata hemocytes on uncoated plates), 1.17±0.004 (MDA-MB-231 breast cancer cells), 1.24±0.006 (SC5 mouse Sertoli cells) and 2.21±0.01 (B. glabrata hemocytes on Poly-L-Lysine coated plates), were measured and are consistent with previous reports. We believe that this system, coupled with open-source analysis software, demonstrates that higher throughput time-lapse imaging of cells for the purpose of studying motility can be an affordable option for all researchers. © 2014 Lynch et al.en_US
dc.languageeng-
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.subjectVisualisation of cellsen_US
dc.subjectMotilityen_US
dc.subjectMicroscopyen_US
dc.titleLow-Cost Motility Tracking System (LOCOMOTIS) for time-lapse microscopy applications and cell visualisationen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0103547-
dc.relation.isPartOfPLoS ONE-
dc.relation.isPartOfPLoS ONE-
pubs.issue8-
pubs.issue8-
pubs.volume9-
pubs.volume9-
pubs.organisational-data/Brunel-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences/Biological Sciences-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme/Institute of Environmental, Health and Societies-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme/Institute of Environmental, Health and Societies/Health and Environment-
pubs.organisational-data/Brunel/Specialist Centres-
pubs.organisational-data/Brunel/Specialist Centres/IfE-
Appears in Collections:Brunel OA Publishing Fund
Dept of Life Sciences Research Papers

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