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|Title:||The clinicopathological significance of lamin A/C, lamin B1 and lamin B receptor mRNA expression in human breast cancer|
|Keywords:||Lamin A/C;Lamin B;Lamin B receptor;Breast cancer;qPCR;Chromosomal instability;Cell senescence;Cell cycle;DNA repair;Ageing|
|Publisher:||Walter de Gruyter GmbH|
|Citation:||Cellular and Molecular Biology Letters, 18(4): 1 - 17, (December 2013)|
|Abstract:||Lamin A/C (LMNA), lamin B1 (LMNB1) and lamin B receptor (LBR) have key roles in nuclear structural integrity and chromosomal stability. In this study, we have studied the relationships between the mRNA expressions of A-type lamins, LMNB1 and LBR and the clinicopathological parameters in human breast cancer. Samples of breast cancer tissues (n = 115) and associated non-cancerous tissue (ANCT; n = 30) were assessed using reverse transcription and quantitative PCR. Transcript levels were correlated with clinicopathological data. Higher levels of A-type lamins and LMNB1 mRNA expression were seen in ANCT. Higher lamin A/C expression was associated with the early clinical stage (TNM1 vs. TNM3 - 13 vs. 0.21; p = 0.0515), with better clinical outcomes (disease-free survival vs. mortality - 11 vs. 1; p = 0.0326), and with better overall (p = 0.004) and disease-free survival (p = 0.062). The expression of LMNB1 declined with worsening clinical outcome (disease-free vs. mortalities - 0.0011 vs. 0.000; p = 0.0177). LBR mRNA expression was directly associated with tumor grade (grade 1 vs. grade 3-0.00 vs. 0.00; p = 0.0479) and Nottingham Prognostic Index (NPI1 vs. NPI3-0.00 vs. 0.00; p = 0.0551). To the best of our knowledge, this is the first study to suggest such a role for A-type lamins, lamin B1 and LBR in human breast cancer, identifying an important area for further research. © 2013 Versita Warsaw and Springer-Verlag Wien.|
|Appears in Collections:||Dept of Life Sciences Research Papers|
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