Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/8910
Title: Trf1 is not required for proliferation or functional telomere maintenance in chicken DT40 cells
Authors: Cooley, C
Baird, KM
Faure, V
Wenner, T
Stewart, JL
Modino, S
Slijepcevic, P
Farr, CJ
Morrison, CG
Keywords: Trf1;Chicken DT40 cells;Telomere;Gernome stability
Issue Date: 2009
Publisher: The American Society for Cell Biology
Citation: Molecular Biology of the Cell, 20(10), 2563 - 2571, 2009
Abstract: The telomere end-protection complex prevents the ends of linear eukaryotic chromosomes from degradation or inappropriate DNA repair. The homodimeric double-stranded DNA-binding protein, Trf1, is a component of this complex and is essential for mouse embryonic development. To define the requirement for Trf1 in somatic cells, we deleted Trf1 in chicken DT40 cells by gene targeting. Trf1-deficient cells proliferated as rapidly as control cells and showed telomeric localization of Trf2, Rap1, and Pot1. Telomeric G-strand overhang lengths were increased in late-passage Trf1-deficient cells, although telomere lengths were unaffected by Trf1 deficiency, as determined by denaturing Southern and quantitative FISH analysis. Although we observed some clonal variation in terminal telomere fragment lengths, this did not correlate with cellular Trf1 levels. Trf1 was not required for telomere seeding, indicating that de novo telomere formation can proceed without Trf1. The Pin2 isoform and a novel exon 4, 5–deleted isoform localized to telomeres in Trf1-deficient cells. Trf1-deficient cells were sensitive to DNA damage induced by ionizing radiation. Our data demonstrate that chicken DT40 B cells do not require Trf1 for functional telomere structure and suggest that Trf1 may have additional, nontelomeric roles involved in maintaining genome stability.
Description: © 2009 by The American Society for Cell Biology.
URI: http://www.molbiolcell.org/content/20/10/2563
http://bura.brunel.ac.uk/handle/2438/8910
DOI: http://dx.doi.org/10.1091/mbc.E08-10-1019
ISSN: 1059-1524
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

Files in This Item:
File Description SizeFormat 
Fulltext.pdf462.67 kBAdobe PDFView/Open


Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.