Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/8358
Title: Oligopeptide-mediated gene transfer into mouse corneal endothelial cells: Expression, design optimization, uptake mechanism and nuclear localization
Authors: Seow, WY
Yang, Y-Y
George, AJT
Keywords: Gene transfer;Corneal transplant;Oligopeptides;T-cells
Issue Date: 2009
Publisher: Oxford University Press
Citation: Nucleic Acids Research, 37(18), 6276 - 6289, 2009
Abstract: Gene transfer to the corneal endothelium has potential in preventing corneal transplant rejection. In this study, we transfected mouse corneal endothelial cells (MCEC) with a class of novel arginine-rich oligopeptides. The peptides featured a tri-block design and mediated reporter gene expression in MCEC more efficiently than the commercial polyethylenimine standard. The functionality of each block was demonstrated to critically influence the performance of the peptide. Results from confocal imaging and flow cytometry then showed that energy-dependent endocytosis was the dominant form of uptake and multiple pathways were involved. Additionally, uptake was strongly dependent on interactions with cell-surface heparan sulphate. Fluorescence resonance energy transfer studies revealed that the peptide/DNA entered cells as an associated complex and some will have dissociated by 8.5 h. Large-scale accumulation of uncondensed DNA within the nucleus can also be observed by 26 h. Finally, as a proof of biological relevance, we transfected MCEC with plasmids encoding for the functional indoleamine 2,3-dioxygenase (IDO) enzyme. We then demonstrated that the expressed IDO could catalyse the degradation of l-tryptophan, which in turn suppressed the growth of CD4+ T-cells in a proliferation assay.
Description: Copyright © The Author 2009. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses?by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://nar.oxfordjournals.org/content/37/18/6276
http://bura.brunel.ac.uk/handle/2438/8358
DOI: http://dx.doi.org/10.1093/nar/gkp651
ISSN: 0305-1048
Appears in Collections:Biological Sciences
Publications
Dept of Life Sciences Research Papers

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