Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/8060
Title: A human coronavirus responsible for the common cold massively kills dendritic cells but not monocytes
Authors: Millet, J
Vidalain, PO
Law, H
Vabret, A
Lorin, V
Escriou, N
Albert, ML
Nal, B
Tangy, F
Keywords: Common Cold;Human coronavirus 229E;Dendritic cells;Monocytes
Issue Date: 2012
Publisher: American Society for Microbiology
Citation: Journal of Virology, 86(14), 7577 - 7587, 2012
Abstract: Human coronaviruses are associated with upper respiratory tract infections that occasionally spread to the lungs and other organs. Although airway epithelial cells represent an important target for infection, the respiratory epithelium is also composed of an elaborate network of dendritic cells (DCs) that are essential sentinels of the immune system, sensing pathogens and presenting foreign antigens to T lymphocytes. In this report, we show that in vitro infection by human coronavirus 229E (HCoV-229E) induces massive cytopathic effects in DCs, including the formation of large syncytia and cell death within only few hours. In contrast, monocytes are much more resistant to infection and cytopathic effects despite similar expression levels of CD13, the membrane receptor for HCoV-229E. While the differentiation of monocytes into DCs in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4 requires 5 days, only 24 h are sufficient for these cytokines to sensitize monocytes to cell death and cytopathic effects when infected by HCoV-229E. Cell death induced by HCoV-229E is independent of TRAIL, FasL, tumor necrosis factor alpha, and caspase activity, indicating that viral replication is directly responsible for the observed cytopathic effects. The consequence of DC death at the early stage of HCoV-229E infection may have an impact on the early control of viral dissemination and on the establishment of long-lasting immune memory, since people can be reinfected multiple times by HCoV-229E.
Description: Copyright @ 2012, American Society for Microbiology.
URI: http://jvi.asm.org/content/86/14/7577
http://bura.brunel.ac.uk/handle/2438/8060
DOI: http://dx.doi.org/10.1128/JVI.00269-12
ISSN: 0022-538X
Appears in Collections:Biological Sciences
Publications
Dept of Life Sciences Research Papers

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