Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/7974
Title: Reduced chromosome aberration complexity in normal human bronchial epithelial cells exposed to low-LET γ-rays and high-LET α-particles
Authors: Themis, M
Garimberti, E
Hill, MA
Anderson, RM
Keywords: M-FISH;Geometry of cell nucleus;Radiation exposure;Human lung
Issue Date: 2013
Publisher: Informa Healthcare
Citation: International Journal of Radiation Biology, 89(11), 934-943, 2013
Abstract: Purpose: Cells of the lung are at risk from exposure to low and moderate doses of ionizing radiation from a range of environmental and medical sources. To help assess human health risks from such exposures, a better understanding of the frequency and types of chromosome aberration initially-induced in human lung cell types is required to link initial DNA damage and rearrangements with transmission potential and, to assess how this varies with radiation quality. Materials and methods: We exposed normal human bronchial lung epithelial (NHBE) cells in vitro to 0.5 and 1 Gy low-linear energy transfer (LET) γ-rays and a low fluence of high-LET α-particles and assayed for chromosome aberrations in premature chromosome condensation (PCC) spreads by 24-color multiplex-fluorescence in situ hybridization (M-FISH). Results: Both simple and complex aberrations were induced in a LET and dose-dependent manner; however, the frequency and complexity observed were reduced in comparison to that previously reported in spherical cell types after exposure to comparable doses or fluence of radiation. Approximately 1–2% of all exposed cells were categorized as being capable of transmitting radiation-induced chromosomal damage to future NHBE cell generations, irrespective of dose. Conclusion: One possible mechanistic explanation for this reduced complexity is the differing geometric organization of chromosome territories within ellipsoid nuclei compared to spherical nuclei. This study highlights the need to better understand the role of nuclear organization in the formation of exchange aberrations and, the influence three-dimensional (3D) tissue architecture may have on this in vivo.
Description: This article is made available through the Brunel Open Access Publishing Fund.
URI: http://informahealthcare.com/doi/abs/10.3109/09553002.2013.805889
http://bura.brunel.ac.uk/handle/2438/7974
DOI: http://dx.doi.org/10.3109/09553002.2013.805889
ISSN: 0955-3002
Appears in Collections:Biological Sciences
Publications
Brunel OA Publishing Fund
Dept of Life Sciences Research Papers

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