Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/7801
Title: Contrasting roles of condensin I and condensin II in mitotic chromosome formation
Authors: Green, LC
Kalitsis, P
Chang, TM
Cipetic, M
Kim, JH
Marshall, O
Turnbull, L
Whitchurch, CB
Vagnarelli, P
Samejima, K
Earnshaw, WC
Choo, KH
Hudson, DF
Keywords: Adenosine Triphosphatases;Animals;Cell Line, Tumor;Chickens;Chromosomes;DNA-Binding Proteins;Gene Knockout Techniques;Mitosis;Multiprotein Complexes
Issue Date: 2012
Publisher: Company of Biologists
Citation: Journal of cell science, 125(6), 1591 - 1604, 2012
Abstract: In vertebrates, two condensin complexes exist, condensin I and condensin II, which have differing but unresolved roles in organizing mitotic chromosomes. To dissect accurately the role of each complex in mitosis, we have made and studied the first vertebrate conditional knockouts of the genes encoding condensin I subunit CAP-H and condensin II subunit CAP-D3 in chicken DT40 cells. Live-cell imaging reveals highly distinct segregation defects. CAP-D3 (condensin II) knockout results in masses of chromatin-containing anaphase bridges. CAP-H (condensin I)-knockout anaphases have a more subtle defect, with chromatids showing fine chromatin fibres that are associated with failure of cytokinesis and cell death. Super-resolution microscopy reveals that condensin-I-depleted mitotic chromosomes are wider and shorter, with a diffuse chromosome scaffold, whereas condensin-II-depleted chromosomes retain a more defined scaffold, with chromosomes more stretched and seemingly lacking in axial rigidity. We conclude that condensin II is required primarily to provide rigidity by establishing an initial chromosome axis around which condensin I can arrange loops of chromatin.
Description: © 2012. Published by The Company of Biologists Ltd
URI: http://www.ncbi.nlm.nih.gov/pubmed/22344259
http://bura.brunel.ac.uk/handle/2438/7801
DOI: http://dx.doi.org/10.1242/jcs.097790
ISSN: 0021-9533
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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