Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/3011
Title: Multiple structural alignment for distantly related all b structures using TOPS pattern discovery and simulated annealing
Authors: Williams, A
Gilbert, D
Westhead, DR
Keywords: Pattern discovery;Protein topology;Simulated annealing;Structural alignment;Superfolds
Issue Date: 2003
Publisher: Oxford University Press
Citation: Protein Engineering. 16(12): 913-923
Abstract: Topsalign is a method that will structurally align diverse protein structures, for example, structural alignment of protein superfolds. All proteins within a superfold share the same fold but often have very low sequence identity and different biological and biochemical functions. There is often signi®cant structural diversity around the common scaffold of secondary structure elements of the fold. Topsalign uses topological descriptions of proteins. A pattern discovery algorithm identi®es equivalent secondary structure elements between a set of proteins and these are used to produce an initial multiple structure alignment. Simulated annealing is used to optimize the alignment. The output of Topsalign is a multiple structure-based sequence alignment and a 3D superposition of the structures. This method has been tested on three superfolds: the b jelly roll, TIM (a/b) barrel and the OB fold. Topsalign outperforms established methods on very diverse structures. Despite the pattern discovery working only on b strand secondary structure elements, Topsalign is shown to align TIM (a/b) barrel superfamilies, which contain both a helices and b strands.
URI: http://bura.brunel.ac.uk/handle/2438/3011
DOI: http://dx.doi.org/10.1093/protein/gzg116
Appears in Collections:Computer Science
Dept of Computer Science Research Papers

Files in This Item:
File Description SizeFormat 
913.pdf624.08 kBAdobe PDFView/Open


Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.