Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/14662
Title: PDGFR alpha demarcates the cardiogenic clonogenic Sca1(+) stem/progenitor cell in adult murine myocardium
Authors: Noseda, M
Harada, M
McSweeney, S
Leja, T
Belian, E
Stuckey, DJ
Paiva, MSA
Habib, J
Macaulay, I
de Smith, AJ
al-Beidh, F
Sampson, R
Lumbers, RT
Rao, P
Harding, SE
Blakemore, AIF
Jacobsen, SE
Barahona, M
Schneider, MD
Keywords: Science & Technology;Multidisciplinary Sciences;Science & Technology - Other Topics;CARDIAC STEM-CELLS;SIDE POPULATION CELLS;PROGENITOR CELLS;SMOOTH-MUSCLE;PRESSURE-OVERLOAD;TRANSGENIC MICE;HEART REPAIR;IN-VITRO;CARDIOMYOCYTES;REGENERATION
Issue Date: 2015
Publisher: NATURE PUBLISHING GROUP
Citation: NATURE COMMUNICATIONS, (2015)
Abstract: Cardiac progenitor/stem cells in adult hearts represent an attractive therapeutic target for heart regeneration, though (inter)-relationships among reported cells remain obscure. Using single-cell qRT–PCR and clonal analyses, here we define four subpopulations of cardiac progenitor/stem cells in adult mouse myocardium all sharing stem cell antigen-1 (Sca1), based on side population (SP) phenotype, PECAM-1 (CD31) and platelet-derived growth factor receptor-a (PDGFRa) expression. SP status predicts clonogenicity and cardiogenic gene expression (Gata4/6, Hand2 and Tbx5/20), properties segregating more specifically to PDGFRaþ cells. Clonal progeny of single Sca1þ SP cells show cardiomyocyte, endothelial and smooth muscle lineage potential after cardiac grafting, augmenting cardiac function although durable engraftment is rare. PDGFRa cells are characterized by Kdr/Flk1, Cdh5, CD31 and lack of clonogenicity. PDGFRaþ/CD31 cells derive from cells formerly expressing Mesp1, Nkx2-5, Isl1, Gata5 and Wt1, distinct from PDGFRa /CD31þ cells (Gata5 low; Flk1 and Tie2 high). Thus, PDGFRa demarcates the clonogenic cardiogenic Sca1þ stem/progenitor cell.
URI: http://bura.brunel.ac.uk/handle/2438/14662
DOI: http://dx.doi.org/10.1038/ncomms7930
ISSN: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000355526900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=f12c8c83318cf2733e615e54d9ed7ad5
ARTN 6930
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000355526900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=f12c8c83318cf2733e615e54d9ed7ad5
ARTN 6930
2041-1723
Appears in Collections:Dept of Life Sciences Research Papers

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