Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/12950
Title: Modular development of a prototype point of care molecular diagnostic platform for sexually transmitted infections
Authors: Branavan, M
Mackay, RE
Craw, P
Naveenathayalan, A
Ahern, JC
Sivanesan, T
Hudson, C
Stead, T
Kremer, J
Garg, N
Baker, M
Sadiq, ST
Balachandran, W
Keywords: Microfluidics;Microengineering;Point-of-care;Molecular diagnostics
Issue Date: 2016
Publisher: Elsevier
Citation: Medical Engineering and Physics, 38(8): pp. 741–748, (2016)
Abstract: This paper presents the design of a modular point of care test platform that integrates a proprietary sample collection device directly with a microfluidic cartridge. Cell lysis, within the cartridge, is conducted using a chemical method and nucleic acid purification is done on an activated cellulose membrane. The microfluidic device incorporates passive mixing of the lysis-binding buffers and sample using a serpentine channel. Results have shown extraction efficiencies for this new membrane of 69% and 57% compared to the commercial Qiagen extraction method of 85% and 59.4% for 0.1ng/μL and 100ng/μL salmon sperm DNA respectively spiked in phosphate buffered solution. Extraction experiments using the serpentine passive mixer cartridges incorporating lysis and nucleic acid purification showed extraction efficiency around 80% of the commercial Qiagen kit. Isothermal amplification was conducted using thermophillic helicase dependant amplification and recombinase polymerase amplification. A low cost benchtop real-time isothermal amplification platform has been developed capable of running six amplifications simultaneously. Results show that the platform is capable of detecting 1.32×106 of sample DNA through thermophillic helicase dependant amplification and 1×105 copy numbers Chlamydia trachomatis genomic DNA within 10min through recombinase polymerase nucleic acid amplification tests.
URI: http://www.sciencedirect.com/science/article/pii/S1350453316300789
http://bura.brunel.ac.uk/handle/2438/12950
DOI: http://dx.doi.org/10.1016/j.medengphy.2016.04.022
ISSN: 1350-4533
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