Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/12823
Title: Centrifugal partition chromatography in a biorefinery context: Separation of monosaccharides from hydrolysed sugar beet pulp
Authors: Ward, DP
Cárdenas-Fernández, M
Hewitson, P
Ignatova, S
Lye, GJ
Keywords: Monosaccharide separation;Sugar beet pulp;Whole crop biorefinery;Hydrolyzed pectin;Centrifugal partition chromatography;Sustainable bio-derived feedstocks
Issue Date: 2015
Publisher: Elsevier
Citation: Journal of Chromatography A, 1411: pp. 84 - 91, (2015)
Abstract: A critical step in the bioprocessing of sustainable biomass feedstocks, such as sugar beet pulp (SBP), is the isolation of the component sugars from the hydrolysed polysaccharides. This facilitates their subsequent conversion into higher value chemicals and pharmaceutical intermediates. Separation methodologies such as centrifugal partition chromatography (CPC) offer an alternative to traditional resin-based chromatographic techniques for multicomponent sugar separations. Highly polar two-phase systems containing ethanol and aqueous ammonium sulphate are examined here for the separation of monosaccharides present in hydrolysed SBP pectin: l-rhamnose, l-arabinose, d-galactose and d-galacturonic acid. Dimethyl sulfoxide (DMSO) was selected as an effective phase system modifier improving monosaccharide separation. The best phase system identified was ethanol:DMSO:aqueous ammonium sulphate (300gL<sup>-1</sup>) (0.8:0.1:1.8, v:v:v) which enabled separation of the SBP monosaccharides by CPC (200mL column) in ascending mode (upper phase as mobile phase) with a mobile phase flow rate of 8mLmin<sup>-1</sup>. A mixture containing all four monosaccharides (1.08g total sugars) in the proportions found in hydrolysed SBP was separated into three main fractions; a pure l-rhamnose fraction (>90%), a mixed l-arabinose/d-galactose fraction and a pure d-galacturonic acid fraction (>90%). The separation took less than 2h demonstrating that CPC is a promising technique for the separation of these sugars with potential for application within an integrated, whole crop biorefinery.
URI: http://www.sciencedirect.com/science/article/pii/S002196731501136X
http://bura.brunel.ac.uk/handle/2438/12823
DOI: http://dx.doi.org/10.1016/j.chroma.2015.08.006
ISSN: 0021-9673
1873-3778
Appears in Collections:Brunel Institute for Bioengineering (BIB)

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