Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/12530
Title: Efficacy of crosslinking on tailoring in vivo biodegradability of fibro-porous decellularized extracellular matrix and restoration of native tissue structure: A quantitative study using stereology methods
Authors: Burugapalli, K
Burugapalli, K
Chan, JCY
Kelly, JL
Pandit, AS
Keywords: Cholecyst-derived extracellular matrix;Crosslinking;Biodegradation;Stereology;Tissue-implant interactions
Issue Date: 2014
Publisher: Wiley
Citation: Macromolecular Bioscience, 14, (2): pp. 244 - 256, (2014)
Abstract: Cholecyst-derived extracellular matrix (CEM) is a fibro-porous decellularized serosal layer of porcine gall-bladder. CEM loses 90% of its weight at 48 h of in vitro collagenase digestion, but takes two months to be completely resorbed in vivo. Carbodiimide (EDC) crosslinking helps tailoring CEM's in vitro collagenase susceptibility. Here, the efficacy of EDC crosslinking on tailoring in vivo biodegradability of CEM is reported. CEM crosslinked with 0.0005 and 0.0033 × 103 M of EDC/mg that lose 80% and 0% of their weight respectively to in vitro collagenase digestion, were present even after 180 days in vivo. Quantitative histopathology using stereology methods confirmed our qualitative observation that even a tiny degree of crosslinking can significantly prolong the rate of in vivo degradation and removal of CEM. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
URI: http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300195/epdf
http://bura.brunel.ac.uk/handle/2438/12530
DOI: http://dx.doi.org/10.1002/mabi.201300195
ISSN: 1616-5187
Appears in Collections:Institute for the Environment

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