Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/12455
Title: Pattern recognition of estradiol, testosterone and dihydrotestosterone in children's saliva samples using stochastic microsensors
Authors: Staden, R-IS-V
Gugoaşă, LA
Calenic, B
Legler, J
Issue Date: 2014
Publisher: Nature Publishing Group
Citation: Scientific Reports, 4, ARTN 5579, (2014)
Abstract: Stochastic microsensors based on diamond paste and three types of electroactive materials (maltodextrin (MD), α-cyclodextrin (α-CD) and 5,10,15,20-tetraphenyl-21H,23H porphyrin (P)) were developed for the assay of estradiol (E2), testosterone (T2) and dihydrotestosterone (DHT) in children's saliva. The main advantage of utilization of such tools is the possibility to identify and quantify all three hormones within minutes in small volumes of childen's saliva. The limits of quantification obtained for DHT, T2, and E2 (1 fmol/L for DHT, 1 pmol/L for T2, and 66 fmol/L for E2) determined using the proposed tools allows the utilization of these new methods with high reliability for the screening of saliva samples from children. This new method proposed for the assay of the three hormones overcomes the limitations (regarding limits of determination) of ELISA method which is the standard method used in clinical laboratories for the assay of DHT, T2, and E2 in saliva samples. The main feature of its utilization for children's saliva is to identify earlier problems related to early puberty and obesity.
URI: http://www.nature.com/articles/srep05579
http://bura.brunel.ac.uk/handle/2438/12455
DOI: http://dx.doi.org/10.1038/srep05579
ISSN: 2045-2322
Appears in Collections:Dept of Life Sciences Research Papers

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