Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/11809
Title: Multicolour correlative imaging using phosphor probes
Authors: Morrison, IEG
Samilian, A
Coppo, P
Ireland, TG
Fern, GR
Silver, J
Withnall, R
O’Toole, PJ
Keywords: Phosphor;Cathodoluminescence;Correlative light and electron microscopy (CLEM);Super-resolution microscopy (SRM);Atmospheric scanning electron microscope (ASEM)
Issue Date: 2015
Publisher: Springer Verlag
Citation: Journal of Chemical Biology, 8 (4): pp. 169 - 177, (2015)
Abstract: Correlative light and electron microscopy exploits the advantages of optical methods, such as multicolour probes and their use in hydrated live biological samples, to locate functional units, which are then correlated with structural details that can be revealed by the superior resolution of electron microscopes. One difficulty is locating the area imaged by the electron beam in the much larger optical field of view. Multifunctional probes that can be imaged in both modalities and thus register the two images are required. Phosphor materials give cathodoluminescence (CL) optical emissions under electron excitation. Lanthanum phosphate containing thulium or terbium or europium emits narrow bands in the blue, green and red regions of the CL spectrum; they may be synthesised with very uniform-sized crystals in the 10- to 50-nm range. Such crystals can be imaged by CL in the electron microscope, at resolutions limited by the particle size, and with colour discrimination to identify different probes. These materials also give emissions in the optical microscope, by multiphoton excitation. They have been deposited on the surface of glioblastoma cells and imaged by CL. Gadolinium oxysulphide doped with terbium emits green photons by either ultraviolet or electron excitation. Sixty-nanometre crystals of this phosphor have been imaged in the atmospheric scanning electron microscope (JEOL ClairScope). This probe and microscope combination allow correlative imaging in hydrated samples. Phosphor probes should prove to be very useful in correlative light and electron microscopy, as fiducial markers to assist in image registration, and in high/super resolution imaging studies.
URI: http://link.springer.com/article/10.1007%2Fs12154-015-0141-5
http://bura.brunel.ac.uk/handle/2438/11809
DOI: http://dx.doi.org/10.1007/s12154-015-0141-5
ISSN: 1864-6158
1864-6166
Appears in Collections:Dept of Mechanical Aerospace and Civil Engineering Research Papers

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