Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/11515
Title: Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation
Authors: Owoka, T
Vetter, M
Federico, C
Saccone, S
Tosi, S
Keywords: Fluorescence in situ hybridisation;t(7;12);HLXB9;ETV6;Chromosome abnormalities;Myeloid malignancies;Paediatric leukaemia
Issue Date: 2015
Publisher: Herbert Publications Limited
Citation: Hematology and Leukemia, 3:4, (2015)
Abstract: The identification of chromosomal rearrangements is of utmost importance for the diagnosis and classification of specific leukaemia subtypes and therefore has an impact on therapy choices in individual cases. The t(7;12)(q36;p13) is a cryptic rearrangement that is difficult to recognise using conventional cytogenetic methods and is often undetected by reverse transcription polymerase chain reaction due to the absence of a fusion transcript in many cases. Here we present a reliable and easy to use dual colour fluorescence in situ hybridisation assay for the detection of the t(7;12)(q36;p13) rearrangement. A comparison with previous similar work is given and advantages and limitations of this novel approach are discussed.
URI: http://www.hoajonline.com/hematolleuk/2052-434X/3/4
http://bura.brunel.ac.uk/handle/2438/11515
DOI: http://dx.doi.org/10.7243/2052-434X-3-4
ISSN: 2052-434X
Appears in Collections:Dept of Life Sciences Research Papers

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