Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/10842
Title: Transcriptional factor PU.1 regulates decidual C1q expression in early pregnancy in human
Authors: Madhukaran, SP
Kishore, U
Jamil, K
Teo, BHD
Choolani, M
Lu, J
Keywords: C1q;Decidua;Implantation;Pregnancy;Stromal cells;Transcription factor;Trophoblast
Issue Date: 2015
Publisher: Frontiers Research Foundation
Citation: Frontiers in Immunology, 6: 53, (16 February 2015)
Abstract: C1q is the first recognition subcomponent of the complement classical pathway, which in addition to being synthesized in the liver, is also expressed by macrophages and dendritic cells (DCs). Trophoblast invasion during early placentation results in accumulation of debris that triggers the complement system. Hence, both early and late components of the classical pathway are widely distributed in the placenta and decidua. In addition, C1q has recently been shown to significantly contribute to feto-maternal tolerance, trophoblast migration, and spiral artery remodeling, although the exact mechanism remains unknown. Pregnancy in mice, genetically deficient in C1q, mirrors symptoms similar to that of human preeclampsia. Thus, regulated complement activation has been proposed as an essential requirement for normal successful pregnancy. Little is known about the molecular pathways that regulate C1q expression in pregnancy. PU.1, an Ets-family transcription factor, is required for the development of hematopoietic myeloid lineage immune cells, and its expression is tissue-specific. Recently, PU.1 has been shown to regulate C1q gene expression in DCs and macrophages. Here, we have examined if PU.1 transcription factor regulates decidual C1q expression. We used immune-histochemical analysis, PCR, and immunostaining to localize and study the gene expression of PU.1 transcription factor in early human decidua. PU.1 was highly expressed at gene and protein level in early human decidual cells including trophoblast and stromal cells. Surprisingly, nuclear as well as cytoplasmic PU.1 expression was observed. Decidual cells with predominantly nuclear PU.1 expression had higher C1q expression. It is likely that nuclear and cytoplasmic PU.1 localization has a role to play in early pregnancy via regulating C1q expression in the decidua during implantation.
Description: "Copyright: © 2015 Madhukaran, Kishore, Jamil, Teo, Choolani and Lu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms."
URI: http://journal.frontiersin.org/article/10.3389/fimmu.2015.00053/full#h1
http://bura.brunel.ac.uk/handle/2438/10842
DOI: http://dx.doi.org/10.3389/fimmu.2015.00053
ISSN: 1664-3224
Appears in Collections:Dept of Life Sciences Research Papers

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