Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/10483
Title: Efficient assembly and secretion of recombinant subviral particles of the four dengue serotypes using native prM and E proteins.
Authors: Wang, PG
Kudelko, M
Lo, J
Siu, LY
Kwok, KT
Sachse, M
Nicholls, JM
Bruzzone, R
Altmeyer, RM
Nal, B
Keywords: Native viral proteins;Codon optimization strategy;Recombinant subviral particles;Dengue virus;Endoplasmic reticulum
Issue Date: 2009
Publisher: PLOS ONE
Citation: PloS ONE, 4(12): e8325, (2009)
Abstract: Background: Flavivirus infected cells produce infectious virions and subviral particles, both of which are formed by the assembly of prM and E envelope proteins and are believed to undergo the same maturation process. Dengue recombinant subviral particles have been produced in cell cultures with either modified or chimeric proteins but not using the native forms of prM and E. Methodology/Principal Findings: We have used a codon optimization strategy to obtain an efficient expression of native viral proteins and production of recombinant subviral particles (RSPs) for all four dengue virus (DV) serotypes. A stable HeLa cell line expressing DV1 prME was established (HeLa-prME) and RSPs were analyzed by immunofluorescence and transmission electron microscopy. We found that E protein is mainly present in the endoplasmic reticulum (ER) where assembly of RSPs could be observed. Biochemical characterization of DV1 RSPs secretion revealed both prM protein cleavage and homodimerization of E proteins before their release into the supernatant, indicating that RSPs undergo a similar maturation process as dengue virus. Pulse chase experiment showed that 8 hours are required for the secretion of DV1 RSPs. We have used HeLa-prME to develop a semi-quantitative assay and screened a human siRNA library targeting genes involved in membrane trafficking. Knockdown of 23 genes resulted in a significant reduction in DV RSP secretion, whereas for 22 others we observed an increase of RSP levels in cell supernatant. Conclusions/Significance: Our data describe the efficient production of RSPs containing native prM and E envelope proteins for all dengue serotypes. Dengue RSPs and corresponding producing cell lines are safe and novel tools that can be used in the study of viral egress as well as in the development of vaccine and drugs against dengue virus.
Description: © 2009 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0008325
http://bura.brunel.ac.uk/handle/2438/10483
DOI: http://dx.doi.org/10.1371/journal.pone.0008325
ISSN: 1932-6203
Appears in Collections:Dept of Life Sciences Research Papers

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