Please use this identifier to cite or link to this item: http://buratest.brunel.ac.uk/handle/2438/10246
Title: Frataxin deficiency increases cyclooxygenase 2 and prostaglandins in cell and animal models of Friedreich's ataxia
Authors: Hayashi, G
Shen, Y
Pedersen, TL
Newman, JW
Pook, M
Cortopassi, G
Keywords: Cyclooxygenase (COX);Friedreich's ataxia (FRDA);Frataxin deficiency;Prostaglandins
Issue Date: 2014
Publisher: Oxford Journals
Citation: Human Molecular Genetics: 1- 10, (August 2014)
Abstract: An inherited deficiency of the mitochondrial protein frataxin causes Friedreich's ataxia (FRDA); the mechanism by which this deficiency triggers neuro- and cardio-degeneration is unclear. Microarrays of neural tissue of animal models of the disease showed decreases in antioxidant genes, and increases in inflammatory genes. Cyclooxygenase (COX)-derived oxylipins are important mediators of inflammation. We measured oxylipin levels using tandem mass spectrometry and ELISAs in multiple cell and animal models of FRDA. Mass spectrometry revealed increases in concentrations of prostaglandins, thromboxane B2, 15-HETE and 11-HETE in cerebellar samples of knockin knockout mice. One possible explanation for the elevated oxylipins is that frataxin deficiency results in increased COX activity. While constitutive COX1 was unchanged, inducible COX2 expression was elevated over 1.35-fold (P < 0.05) in two Friedreich's mouse models and Friedreich's lymphocytes. Consistent with higher COX2 expression, its activity was also increased by 58% over controls. COX2 expression is driven by multiple transcription factors, including activator protein 1 and cAMP response element-binding protein, both of which were elevated over 1.52-fold in cerebella. Taken together, the results support the hypothesis that reduced expression of frataxin leads to elevation of COX2-mediated oxylipin synthesis stimulated by increases in transcription factors that respond to increased reactive oxygen species. These findings support a neuroinflammatory mechanism in FRDA, which has both pathomechanistic and therapeutic implications.
Description: © The Author 2014. Published by Oxford University Press This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hmg.oxfordjournals.org/content/23/25/6838
http://bura.brunel.ac.uk/handle/2438/10246
DOI: http://dx.doi.org/10.1093/hmg/ddu407
ISSN: 1460-2083
Appears in Collections:Dept of Life Sciences Research Papers

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